Journal: Cell reports

Article Title: Pro-survival signaling regulates lipophagy essential for multiple myeloma resistance to stress-induced death

doi: 10.1016/j.celrep.2024.114445

Figure Lengend Snippet: (A) Kaplan-Meier survival analysis on NDMM patients. Details in . (B) LIPA expression from NDMM vs. RRMM patients (left) or patients at diagnosis vs. first relapse (right). Student’s t test for statistical differences. (C) MM were treated with control (Ctrl) or Lal2 in FM for 6 h and imaged by ImageStream (40×). (D and E) MM cells were treated with control (Ctrl) or 10 nM bafilomycin A (BafA) in FM for 6 h and imaged by confocal microscopy (400×) or ImageStream (40×), respectively. Representative photographs in (D) LD/autophagosome similarity score were determined by ImageStream (Exp) in (E). Single color-stained group as negative control (Neg_Ctrl). (F) Mito Stress test of KMS11 in FM ± 50 μM Lal2 ± 10 μM OA for 16 h. (C–F) Representative data from 2 replicates. Student’s t test. ns, not significant; * p ≤ 0.05; ** p ≤ 0.01; *** p ≤ 0.001; **** p ≤ 0.0001.

Article Snippet: Bafilomycin A1 , Tocris , Cat#1334.

Techniques: Expressing, Biomarker Discovery, Control, Confocal Microscopy, Staining, Negative Control

Journal: Cell reports

Article Title: Pro-survival signaling regulates lipophagy essential for multiple myeloma resistance to stress-induced death

doi: 10.1016/j.celrep.2024.114445

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Bafilomycin A1 , Tocris , Cat#1334.

Techniques: Ubiquitin Proteomics, Recombinant, Staining, SYBR Green Assay, cDNA Synthesis, Clinical Proteomics, Cell Isolation, Isolation, Control, shRNA, Plasmid Preparation, Software

Journal: Scientific Reports

Article Title: The local anesthetic, lidocaine, suppresses the growth of colon cancer SW480 cells by decreasing the voltage-gated sodium channel subunit Na v 1.5

doi: 10.1038/s41598-025-29505-1

Figure Lengend Snippet: Na v 1.5 levels decreased in lidocaine-treated SW480 cells. ( A ) Na v 1.5 levels decreased in lidocaine-treated SW480 cells. SW480 cells were treated with or without 5 mM lidocaine for 24 h, and the cell lysates were subjected to western blot analysis using polyclonal anti-Na v 1.5 antibodies. The arrow indicates Na v 1.5. The relative expression levels of Na v 1.5 were normalized to GAPDH levels in each sample. The results of each sample assayed in triplicate are presented as the mean ± SD. * p < 0.05. ( B ) The level of Na v 1.5 did not increase after treatment with a proteasome inhibitor, MG 132, in lidocaine-treated SW480 cells. SW480 cells were treated with 5 mM lidocaine, together with or without 5 µM MG132 for 24 h. The cell lysates were subjected to western blot analysis using polyclonal anti-Na v 1.5 and p21 antibodies. p21 was used as a positive control of MG132 treatment . The arrow indicates Na v 1.5. The relative expression levels of Na v 1.5 and p21 were normalized to a-tubulin levels in each sample. The results of each sample assayed in triplicate are presented as the mean ± SD. * p < 0.05, ** p < 0.01. ( C ) The level of Na v 1.5 did not increase after treatment with an autophagy inhibitor, Bafilomycin A1, in lidocaine-treated SW480 cells. SW480 cells were treated with 5 mM lidocaine with or without 200 nM Bafilomycin A1 (BAF) for 24 h . The arrow indicates Na v 1.5. The relative expression levels of Na v 1.5 and p62 were normalized to a-tubulin levels in each sample. p62 was used as a positive control of Bafilomycin A1 treatment. The results of each sample assayed in triplicate are presented as the mean ± SD. * p < 0.05, ** p < 0.01. ( D ) Na v 1.5 levels in the membrane fraction decreased in lidocaine-treated SW480 cells. SW480 cells were treated with or without 5 mM lidocaine for 24 h, and cell lysates of the cytosol and membrane fractions were prepared using Mem-PER™ Plus Membrane Protein Extraction Kit. Each cell lysate was subjected to western blot analysis. ATP1A1 was used as the membrane marker protein and GDF-15 was used as the lidocaine-inducible protein, which increased in the membrane fraction after lidocaine treatment. The relative expression levels of Na v 1.5 and GDF-15 were normalized to ATP1A1 levels in each sample. The results of each sample assayed in triplicate are presented as the mean ± SD. ** p < 0.01.

Article Snippet: MG132 and Bafilomycin A1 were purchased from Fujifilm-Wako (Osaka, Japan) and LKT Laboratories (MN, USA), respectively.

Techniques: Western Blot, Expressing, Positive Control, Membrane, Protein Extraction, Marker